PCR Detection of <i>Bartonella</i> spp. and <i>Borreliella</i> spp. DNA in Dry Blood Spot Samples from Human Patients.

ABSTRACT: Lyme disease is the most commonly reported vector-borne disease in the United States. Bartonella constitute an additional zoonotic pathogen whose public health impact and diversity continue to emerge. Rapid, sensitive, and specific detection of these and other vector-borne pathogens remains challenging, especially for patients with persistent infections. This report describes an approach for DNA extraction and PCR testing for the detection of Bartonella spp. and Borreliella spp. from dry blood spot (DBS) specimens from human patients. The present study included extraction of DNA and PCR testing of DBS samples from 105 patients with poorly defined, chronic symptoms labeled as Lyme-Like Syndromic Illness (LLSI). Bartonella spp. DNA was detected in 20/105 (19%) and

SUBMITTER: Clark KL 

PROVIDER: S-EPMC11435347 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature