Enhancing Bioluminescence Imaging of Cultured Tissue Explants Using Optical Telecompression.

ABSTRACT: Long-term observation of single-cell oscillations within tissue networks is now possible by combining bioluminescence reporters with stable tissue explant culture techniques. This method is particularly effective in revealing the network dynamics in systems with slow oscillations, such as circadian clocks. However, the low intensity of luciferase-based bioluminescence requires signal amplification using specialized cameras (e.g., I-CCDs and EM-CCDs) and prolonged exposure times, increasing baseline noise and reducing temporal resolution. To address this limitation, we implemented a cost-effective optical enhancement technique called telecompression, first used in astrophotography and now commonly used in digital photography. By combining a high numerical aperture objective lens with a magn

SUBMITTER: Myung J 

PROVIDER: S-EPMC11436007 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature