CRISPR/Cas9-Mediated Resistance to Wheat Dwarf Virus in Hexaploid Wheat (<i>Triticum aestivum</i> L.).

ABSTRACT: Wheat dwarf virus (WDV, genus Mastrevirus, family Geminiviridae) is one of the causal agents of wheat viral disease, which severely impacts wheat production in most wheat-growing regions in the world. Currently, there is little information about natural resistance against WDV in common wheat germplasms. CRISPR/Cas9 technology is being utilized to manufacture transgenic plants resistant to different diseases. In the present study, we used the CRISPR/Cas9 system targeting overlapping regions of coat protein (CP) and movement protein (MP) (referred to as CP/MP) or large intergenic region (LIR) in the wheat variety 'Fielder' to develop resistance against WDV. WDV-inoculated T₁ progenies expressing Cas9 and sgRNA for CP/MP and LIR showed complete resistance against WDV

SUBMITTER: Yuan X 

PROVIDER: S-EPMC11436044 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature