The Diagnostic Value of qPCR Quantification of Paenibacillus larvae in Hive Debris and Adult Bees for Predicting the Onset of American Foulbrood.

ABSTRACT: American foulbrood (AFB) is a serious infectious disease of honeybees (Apis mellifera) caused by Paenibacillus larvae. Increased P. larvae count in hive-related material is associated with an increased risk of AFB. Here, we quantified P. larvae cells in 106 adult bee and 97 hive debris samples using quantitative PCR (qPCR); 66/106 adult bee and 66/97 hive debris samples were collected simultaneously from the same bee colony (paired-sample design). The corresponding bee colonies were also examined for the presence of AFB clinical signs. A binary logistic regression model to distinguish between AFB-affected and unaffected honeybee colonies showed a strong diagnostic accuracy of both sample types for predicting the onset of AFB based on P. larvae counts dete

SUBMITTER: Papic B 

PROVIDER: S-EPMC11436083 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature