The First Isolation of Insect-Specific Alphavirus (<i>Agua Salud alphavirus</i>) in Culex (Melanoconion) Mosquitoes in the Brazilian Amazon.

ABSTRACT: Advances in diagnostic techniques coupled with ongoing environmental changes have resulted in intensified surveillance and monitoring of arbovirus circulation in the Amazon. This increased effort has resulted in increased detection of insect-specific viruses among hematophagous arthropods collected in the field. This study aimed to document the first isolation of Agua Salud alphavirus in mosquitoes collected within the Brazilian Amazon. Arthropods belonging to the family Culicidae were collected within a forest fragment located in the Environmental Protection Area of the metropolitan region of Belem. Subsequently, these specimens were meticulously identified to the species level. Afterward, the collected batches were macerated, and the resulting supernatant was then inoculated into

SUBMITTER: Ramos B 

PROVIDER: S-EPMC11436152 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature