Super-resolved highly multiplexed immunofluorescence imaging for precise protein localization and podocyte ultrastructure.

ABSTRACT: Deep insights into the complex cellular and molecular changes occurring during (patho-)physiological conditions are essential for understanding the interactions and regulation of proteins. This understanding is crucial for research and diagnostics. However, the effectiveness of conventional immunofluorescence and light microscope, tools for visualizing the spatial distribution of cells or proteins, are limited both in resolution and multiplexity in complex tissues. This is mainly due to challenges such as the spectral overlap of fluorophore wavelengths, a limited range of antibody types, the inherent variability of samples and the optical resolution limit. The herein demonstrated combination of multiplex immunofluorescence imaging and super resolution microscopy offers a solution to these

SUBMITTER: Siegerist F 

PROVIDER: S-EPMC11436374 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature