Two-photon optogenetics-based assessment of neuronal connectivity in healthy and chronic hypoperfusion mice.

ABSTRACT:

Significance

Two-photon optogenetics and simultaneous calcium imaging can be used to visualize the response of surrounding neurons with respect to the activity of an optically stimulated target neuron, providing a direct method to assess neuronal connectivity.

Aim

We aim to develop a two-photon optogenetics-based method for evaluating neuronal connectivity, compare it to the existing indirect resting-state synchrony method, and investigate the application of the method to brain pathophysiology.

Approach

C1V1-mScarlet was introduced into GCaMP6s-expressing transgenic mice with an adeno-associated virus. Optical stimulation of a single target neuron and simultaneous calcium imaging of the target and surrounding cells were performed. Neuronal connectivity was evaluated f

SUBMITTER: Yoshioka M 

PROVIDER: S-EPMC11436461 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature