Mitigating bisphenol A-induced apoptosis in KGN cells: the therapeutic role of 1,25-dihydroxyvitamin D₃ through upregulation of PGC-1α expression and inhibition of the mitochondrial cytochrome c pathway.

ABSTRACT:

Purpose

This study investigated the potential of 1,25-dihydroxyvitamin D₃ (1,25(OH)₂VD₃) to mitigate bisphenol A (BPA)-induced apoptosis in human ovarian granulosa KGN cells with the aim of establishing a theoretical foundation for understanding of how vitamin D improved ovarian function in patients with polycystic ovary syndrome (PCOS).

Methods

The impact of varying concentrations of BPA and 1,25(OH)₂VD₃ on KGN cell viability was elucidated. It was established that BPA-induced apoptosis in KGN cells. Subsequently, KGN cells underwent pretreatment with 1,25(OH)₂VD₃, followed by exposure to BPA. The apoptosis rate, reactive oxygen species (ROS) levels, and mitochondrial function of the cells were met

SUBMITTER: Tang L 

PROVIDER: S-EPMC11436470 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature