Supercooling preservation of vascularized composite allografts through CPA optimization, thermal tracking, and stepwise loading techniques.

ABSTRACT: Vascularized composite allografts (VCAs) present unique challenges in transplant medicine, owing to their complex structure and vulnerability to ischemic injury. Innovative preservation techniques are crucial for extending the viability of these grafts, from procurement to transplantation. This study addresses these challenges by integrating cryoprotectant agent (CPA) optimization, advanced thermal tracking, and stepwise CPA loading strategies within an ex vivo rodent model. CPA optimization focused on various combinations, identifying those that effectively suppress ice nucleation while mitigating cytotoxicity. Thermal dynamics were monitored using invasive thermocouples and non-invasive FLIR imaging, yielding detailed temperature profiles crucial for managing warm ischemia time and optim

SUBMITTER: Filz von Reiterdank I 

PROVIDER: S-EPMC11436631 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature