Impact of salt strength on in vitro propagation and rebaudioside A content in Stevia rebaudiana under semi-solid and liquid MS media.

ABSTRACT: Stevia rebaudiana (Bertoni), commonly known as stevia, is a sought-after natural sweetener, but its conventional propagation methods are slow and inefficient. This study aims to enhance the in vitro culture for stevia by investigating the impact of different Murashige and Skoog (MS) medium salt strengths and plant growth hormones on growth and rebaudioside A content. Apical bud-containing shoot segments were used as explants and cultured on various semi-solid and liquid MS media formulations, incorporating cytokinins (BAP and Kin), auxins (NAA and IAA), and different MS major salt concentrations (MS full, ½ MS, and ¼ MS). Assessments of shoot growth parameters, root formation, and HPLC analysis for rebaudioside A content were conducted. The optimal conditions for in vitro growth was found

SUBMITTER: Biswas P 

PROVIDER: S-EPMC11436641 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature