Non-invasive label-free imaging analysis pipeline for in situ characterization of 3D brain organoids.

ABSTRACT: Brain organoids provide a unique opportunity to model organ development in a system similar to human organogenesis in vivo. Brain organoids thus hold great promise for drug screening and disease modeling. Conventional approaches to organoid characterization predominantly rely on molecular analysis methods, which are expensive, time-consuming, labor-intensive, and involve the destruction of the valuable three-dimensional (3D) architecture of the organoids. This reliance on end-point assays makes it challenging to assess cellular and subcellular events occurring during organoid development in their 3D context. As a result, the long developmental processes are not monitored nor assessed. The ability to perform non-invasive assays is critical for longitudinally assessing features of organoid d

SUBMITTER: Serafini CE 

PROVIDER: S-EPMC11436713 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature