Spatial organization of adenylyl cyclase and its impact on dopamine signaling in neurons.

ABSTRACT: The cAMP cascade is increasingly recognized to transduce physiological effects locally through spatially limited cAMP gradients. However, little is known about how adenylyl cyclase enzymes that initiate cAMP gradients are localized. Here we address this question in physiologically relevant striatal neurons and investigate how AC localization impacts downstream signaling function. We show that the major striatal AC isoforms are differentially sorted between ciliary and extraciliary domains of the plasma membrane, and that one isoform, AC9, is uniquely concentrated in endosomes. We identify key sorting determinants in the N-terminal cytoplasmic domain responsible for isoform-specific localization. We further show that AC9-containing endosomes accumulate activated dopamine receptors and form

SUBMITTER: Ripoll L 

PROVIDER: S-EPMC11436756 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature