Histone marks identify novel transcription factors that parse CAR-T subset-of-origin, clinical potential and expansion.

ABSTRACT: Chimeric antigen receptor-modified T cell (CAR-T) immunotherapy has revolutionised blood cancer treatment. Parsing the genetic underpinnings of T cell quality and CAR-T efficacy is challenging. Transcriptomics inform CAR-T state, but the nature of dynamic transcription during activation hinders identification of transiently or minimally expressed genes, such as transcription factors, and over-emphasises effector and metabolism genes. Here we explore whether analyses of transcriptionally repressive and permissive histone methylation marks describe CAR-T cell functional states and therapeutic potential beyond transcriptomic analyses. Histone mark analyses improve identification of differences between naïve, central memory, and effector memory CD8 + T cell subsets of human origin, and CAR-T d

SUBMITTER: Fiorenza S 

PROVIDER: S-EPMC11436946 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature