Massive multiplexing of spatially resolved single neuron projections with axonal BARseq.

ABSTRACT: Neurons in the cortex are heterogeneous, sending diverse axonal projections to multiple brain regions. Unraveling the logic of these projections requires single-neuron resolution. Although a growing number of techniques have enabled high-throughput reconstruction, these techniques are typically limited to dozens or at most hundreds of neurons per brain, requiring that statistical analyses combine data from different specimens. Here we present axonal BARseq, a high-throughput approach based on reading out nucleic acid barcodes using in situ RNA sequencing, which enables analysis of even densely labeled neurons. As a proof of principle, we have mapped the long-range projections of >8000 primary auditory cortex neurons from a single male mouse. We identified major cell types based on projecti

SUBMITTER: Yuan L 

PROVIDER: S-EPMC11437104 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature