DdPCR for the Detection and Absolute Quantification of Oropouche Virus.

ABSTRACT:

Background

Oropouche virus (OROV) is a segmented RNA virus belonging to the genus Orthobunyavirus in the family Peribunyaviridae. Herein, an in-house droplet digital PCR (ddPCR) assay was used for the detection and quantification of OROV.

Methods

The ddPCR reaction was assessed as duplex assay using the human housekeeping gene RPP30. Limit of detection (LoD) analysis was performed in whole blood, serum, and urine. The assay was executed on a total of 28 clinical samples (whole blood n = 9, serum n = 11, and urine n = 8), of which 16 specimens were tested positive at the routine molecular diagnostics (endpoint and real-time PCRs).

Results

The LoD of the ddPCR performed using 10-fold serial dilution of OROV detected up to 1 cp/µL in all the biologic

SUBMITTER: Pomari E 

PROVIDER: S-EPMC11437440 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature