N6-Methyladenosine Positively Regulates Coxsackievirus B3 Replication.

ABSTRACT: Enteroviruses such as coxsackievirus B3 are identified as a common cause of viral myocarditis, but the potential mechanism of its replication and pathogenesis are largely unknown. The genomes of a variety of viruses contain N6-methyladenosine (m⁶A), which plays important roles in virus replication. Here, by using the online bioinformatics tools SRAMP and indirect immunofluorescence assay (IFA), we predict that the CVB3 genome contains m⁶A sites and found that CVB3 infection could alter the expression and cellular localization of m⁶A-related proteins. Moreover, we found that 3-deazaadenosine (3-DAA), an m⁶A modification inhibitor, significantly decreased CVB3 replication. We also observed that the m⁶A methyltransferases methyltransferas

SUBMITTER: Zhao H 

PROVIDER: S-EPMC11437462 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature