Lipid vesicle formation by encapsulation of SMALPs in surfactant-stabilised droplets.

ABSTRACT: Understanding the intricate functions of membrane proteins is pivotal in cell biology and drug discovery. The composition of the cell membrane is highly complex, with different types of membrane proteins and lipid species. Hence, studying cellular membranes in a complexity-reduced context is important to enhance our understanding of the roles of these different elements. However, reconstitution of membrane proteins in an environment that closely mimics the cell, like giant unilamellar vesicles (GUVs), remains challenging, often requiring detergents that compromise protein function. To address this challenge, we present a novel strategy to manufacture GUVs from styrene maleic acid lipid particles (SMALPs) that utilises surfactant-stabilised droplets as a template. As a first step towards th

SUBMITTER: Waeterschoot J 

PROVIDER: S-EPMC11437848 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature