Induction of human stem cells into ameloblasts by reaggregation strategy.

ABSTRACT:

Background

Human epithelium-derived stem cells and induced pluripotent stem cells (hiPSCs) possess the capability to support tooth formation and differentiate into functional enamel-secreting ameloblasts, making them promising epithelial-component substitutes for future human tooth regeneration. However, current tissue recombination approaches are not only technically challenging, requiring precise induction procedures and sophisticated microsurgery, but also exhibit low success rates in achieving tooth formation and ameloblastic differentiation.

Methods

Suspended human keratinocyte stem cells (hKSCs) or cells from three hiPSC lines were directly mixed with dissociated embryonic mouse dental mesenchymal cells (mDMCs) that possess odontogenic potential in different proportion

SUBMITTER: Lin C 

PROVIDER: S-EPMC11437913 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature