Antibacterial periodontal ligament stem cells enhance periodontal regeneration and regulate the oral microbiome.

ABSTRACT:

Background

The transplantation of periodontal ligament stem cells (PDLSCs) has been shown to enhance periodontal regeneration in animal models and clinical trials. However, it is not known whether PDLSCs are antibacterial and whether this affects oral microbiota and periodontal regeneration.

Methods

We isolated human PDLSCs from periodontal ligament of extracted teeth. Rats' periodontal fenestration defects were prepared, and treated with PDLSC injections (Cell group), using saline injections (Saline group) as the control. The oral microbiota was explored by 16 S rDNA sequencing and compared with that before surgery (PRE group). The antibacterial property of PDLSCs and its underlying mechanism were tested in vitro.

Results

Microbiome analyses reveal a decreased biodiv

SUBMITTER: You J 

PROVIDER: S-EPMC11437971 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature