Novel endothelial progenitor cells populations as biomarkers of damage and remission in systemic lupus erythematosus.

ABSTRACT:

Introduction

Endothelial progenitor cells (EPCs) are essential for maintenance of vascular homeostasis and stability, key processes in the pathogenesis of systemic lupus erythematosus (SLE). However, the role and phenotypic characterization of EPCs populations in SLE have not been completely elucidated.

Objective

To identify EPCs specific subpopulations in patients with SLE using a novel flow cytometry tool.

Methods

Peripheral blood mononuclear cells (PBMCs) were isolated from patients with SLE and healthy controls (HC). mRNA and surface protein expression were determined by quantitative PCR (qPCR) and flow cytometry. Clusters identification and characterization were performed using tSNE-CUDA dimensionality reduction algorithms.

Results

tSNE-CUDA analysis ident

SUBMITTER: Rafael-Vidal C 

PROVIDER: S-EPMC11438060 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature