Development and validation of a droplet digital PCR assay for Nipah virus quantitation.

ABSTRACT:

Background

Nipah virus (NiV) is a zoonotic pathogen that poses a significant threat because of its wide host range, multiple transmission modes, high transmissibility, and high mortality rates, affecting both human health and animal husbandry. In this study, we developed a one-step reverse transcription droplet digital PCR (RT-ddPCR) assay that targets the N gene of NiV.

Results

Our RT-ddPCR assay exhibited remarkable sensitivity, with a lower limit of detection of 6.91 copies/reaction. Importantly, it displayed no cross-reactivity with the other 13 common viruses and consistently delivered reliable results with a coefficient of variation below 10% across different concentrations. To validate the effectiveness of our RT-ddPCR assay, we detected 75 NiV armored RNA virus sampl

SUBMITTER: Shuai J 

PROVIDER: S-EPMC11438125 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature