Engineering quorum sensing-based genetic circuits enhances growth and productivity robustness of industrial E. coli at low pH.

ABSTRACT:

Background

Microbial organisms hold significant potential for converting renewable substrates into valuable chemicals. Low pH fermentation in industrial settings offers key advantages, including reduced neutralizer usage and decreased wastewater generation, particularly in the production of amino acids and organic acids. Engineering acid-tolerant strains represents a viable strategy to enhance productivity in acidic environments. Synthetic biology provides dynamic regulatory tools, such as gene circuits, facilitating precise expression of acid resistance (AR) modules in a just-in-time and just-enough manner.

Results

In this study, we aimed to enhance the robustness and productivity of Escherichia coli, a workhorse for amino acid and organic acid production, in industrial fer

SUBMITTER: Yan X 

PROVIDER: S-EPMC11438209 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature