Increased bone mass but delayed mineralization: in vivo and in vitro study for zoledronate in bone regeneration.

ABSTRACT:

Background

Bisphosphonates (BPs) are widely used to inhibit excessive osteoclast activity. However, the potential to compromise bone defect healing has limited their broader application. To better understand the influence of BPs on bone regeneration, we established a bone grafting model with Zoledronate administration, aiming to deepen the understanding of bone remodeling and mineralization processes.

Methods

A bone grafting model was established in the distal femurs of male Sprague-Dawley rats. The experimental group received systemic administration of Zoledronate (ZOL, 0.2 mg/kg, administered twice). Histological analysis and immunohistochemistry (IHC) were employed to assess osteoblastic and macrophage activity, tartrate-resistant acid phosphatase (TRAP) staining was used

SUBMITTER: Wang R 

PROVIDER: S-EPMC11438265 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature