Macrophages Promote Atherosclerosis Development by Inhibiting CD8T Cell Apoptosis.

ABSTRACT:

Background

Atherosclerosis is an inflammatory cardiovascular disease. However, whether the association of immune cells in plaques promotes the progression of this disease has not yet been completely elucidated.

Materials and methods

Thus, this study aimed to investigate the relationship between C1q+ macrophages and CD8T cells through scRNA-seq data reanalysis, quantitative real-time PCR, and flow cytometry. Chromatin immunoprecipitation-quantitative polymerase chain reaction, western blot, and antibody-blocking experiments were performed to investigate the role of macrophage-CD8T interaction in atherosclerosis. An atherosclerotic mouse model was developed to confirm our findings.

Results

Mechanistically, Spi1 expression induced by granulocyte-macrophage colony-stimula

SUBMITTER: Xu X 

PROVIDER: S-EPMC11438514 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature