Suppressing the background of LC-ESI-MS analysis of permethylated glycans using the active background ion reduction device.

ABSTRACT: Mass spectrometry (MS) has revolutionized analytical chemistry, enabling precise identification and quantification of chemical species, which is pivotal for biomarker discovery and understanding complex biological systems. Despite its versatility, the presence of background ions in MS analysis hinders the sensitive detection of low-abundance analytes. Therefore, studies aimed at lowering background ion levels have become increasingly important. Here, we utilized the commercially available Active Background Ion Reduction Device (ABIRD) to suppress background ions and assess its effect on the liquid chromatography-electrospray ionization (LC-ESI)-MS analyses of N-glycans on the Q Exactive HF mass spectrometer. We also investigated the effect of different solvent vapors in the ESI source on N

SUBMITTER: Fowowe M 

PROVIDER: S-EPMC11438568 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature