Blockade of Crk eliminates Yki/YAP-activated tumors via JNK-mediated apoptosis in Drosophila.

ABSTRACT: Selective elimination of cancer cells without causing deleterious effects on normal cells is an ideal anti-cancer strategy. Here, using Drosophila cancer model, we performed an in vivo RNAi screen for anti-cancer targets that selectively eliminate tumors without affecting normal tissue growth. In Drosophila imaginal epithelium, clones of cells expressing oncogenic Ras with simultaneous mutations in the cell polarity gene scribble (Ras^V12/scrib^-/-) develop into malignant tumors. We found that knockdown of Crk, the Drosophila ortholog of human CRK (CT10 regulatory kinase) and CRKL (Crk-like) adapter proteins, significantly suppresses growth of Ras^V12/scrib^-/- tumors by inducing c-Jun N-terminal kinase (JNK)-mediated apoptosis, while it does not aff

SUBMITTER: Kakemura B 

PROVIDER: S-EPMC11438906 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature