Computational immune synapse analysis reveals T-cell interactions in distinct tumor microenvironments.

ABSTRACT: The tumor microenvironment (TME) and the cellular interactions within it can be critical to tumor progression and treatment response. Although technologies to generate multiplex images of the TME are advancing, the many ways in which TME imaging data can be mined to elucidate cellular interactions are only beginning to be realized. Here, we present a novel approach for multipronged computational immune synapse analysis (CISA) that reveals T-cell synaptic interactions from multiplex images. CISA enables automated discovery and quantification of immune synapse interactions based on the localization of proteins on cell membranes. We first demonstrate the ability of CISA to detect T-cell:APC (antigen presenting cell) synaptic interactions in two independent human melanoma imaging mass cytometr

SUBMITTER: Wang VG 

PROVIDER: S-EPMC11438971 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature