Klebsiella pneumoniae AI-2 transporters mediate interspecies interactions and composition in a three-species biofilm community.

ABSTRACT: Biofilms in nature often exist as communities. In this study, an experimental mixed-species community consisting of Pseudomonas aeruginosa, Pseudomonas protegens and Klebsiella pneumoniae was used to investigate how AI-2 transporters affect interspecies interactions and composition. The K. pneumoniae lsrB/lsrD deletion mutants had a 10-25-fold higher concentration of extracellular AI-2 compared to the wild-type. Although these deletion mutants produced monospecies biofilms of similar biomass, the substitution of these mutants for the parental strain significantly altered composition. Dual-species biofilm assays demonstrated that the changes in composition were due to the cumulative effect of pairwise interactions. It was further revealed that K. pneumoniae being present physically in the c

SUBMITTER: Bin Mohammad Muzaki MZ 

PROVIDER: S-EPMC11439081 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature