Single-cell transcriptomics reveals e-cigarette vapor-induced airway epithelial remodeling and injury.

ABSTRACT:

Background

In recent years, e-cigarettes have been used as alternatives among adult smokers. However, the impact of e-cigarette use on human bronchial epithelial (HBE) cells remains controversial.

Methods

We collected primary HBE cells of healthy nonsmokers and chronic obstructive pulmonary disease (COPD) smokers, and analyzed the impact of e- cigarette vapor extract (ECE) or cigarette smoke extract (CSE) on HBE cell differentiation and injury by single-cell RNA sequencing, immunostaining, HE staining, qPCR and ELISA. We obtained serum and sputum from healthy non- smokers, smokers and e-cigarette users, and analyzed cell injury markers and mucin proteins.

Results

ECE treatment led to a distinct differentiation program of ciliated cells and unique patterns of their cel

SUBMITTER: Cao W 

PROVIDER: S-EPMC11439300 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature