ADEVO: Proof-of-concept of adenovirus-directed EVOlution by random peptide display on the fiber knob.

ABSTRACT: Directed evolution of viral vectors involves the generation of randomized libraries followed by artificial selection of improved variants. Directed evolution only yielded limited results in adenovirus (AdV) engineering until now, mainly due to insufficient complexities of randomized libraries. Meanwhile, clinical applications of AdVs as gene therapy or oncolytic vectors are still hampered by the predetermined tropism of natural types. To overcome this challenge, we hypothesized that randomized peptide insertions on the capsid surface can be incorporated into the AdV bioengineering toolbox for retargeting. Here we developed AdV-directed EVOlution protocols based on fiber knob peptide display. Human AdV-C5-derived libraries were constructed following three distinct protocols and selected on

SUBMITTER: Sallard E 

PROVIDER: S-EPMC11439537 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature