Urolithin A alleviates cell senescence by inhibiting ferroptosis and enhances corneal epithelial wound healing.

ABSTRACT:

Purpose

To analyze the therapeutic effect and mechanism of Urolithin A (UA) on delayed corneal epithelial wound healing.

Methods

The C57BL/6 mice were continuously exposed to hyperosmotic stress (HS) for 7 days followed by the removal of central corneal epithelium to establish a delayed corneal epithelial wound healing model in vivo. In vitro, the human corneal epithelial cell line (HCE-T) was also incubated under HS. UA was administered in vivo and in vitro to study its effects on corneal epithelial cells. Senescence-associated β-galactosidase (SA-β-gal) staining was performed to detect the level of cell senescence. Transcriptome sequencing (RNA-seq) was conducted to elucidate the molecular mechanism underlying the effect of UA on corneal epithel

SUBMITTER: Guo XX 

PROVIDER: S-EPMC11439666 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature