Regulatory CD4⁺ T cells redirected against pathogenic CD8⁺ T cells protect NOD mice from development of autoimmune diabetes.

ABSTRACT:

Introduction

In this study, we report a novel therapeutic approach redirecting antigen-specific CD4⁺ T cells recognizing a hybrid insulin peptide (BDC2.5 T cell receptor (TCR) transgenic CD4⁺ T cells) to attract and suppress islet-specific CD8⁺ T cells T cells in the non-obese diabetic (NOD) mouse model, and prevent the development of autoimmune diabetes.

Methods

Purified BDC2.5 CD4⁺ T cells were induced to differentiate into regulatory T cells (Tregs). The Tregs were then electroporated with mRNA encoding chimeric human β₂ microglobulin (hβ₂m) covalently linked to insulin B chain amino acids 15-23 (designated INS-eTreg) or islet-specific glucose-6-phosphatase related protein (IGRP) peptide 206-214 (designated

SUBMITTER: Kakabadse D 

PROVIDER: S-EPMC11439686 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature