Impact of mAb-FcRn affinity on IgG transcytosis across human well-differentiated airway epithelium.

ABSTRACT: Effective treatment and immunoprophylaxis of viral respiratory infections with neutralizing monoclonal antibodies (mAbs) require maintaining inhibitory concentrations of mAbs at the airway surface. While engineered mAbs with increased affinity to the neonatal Fc receptor (FcRn) are increasingly employed, little is known how increased affinity of Fc to FcRn influences basal-to-apical transepithelial transport (transcytosis) of mAbs across the airway epithelium. To investigate this, we utilized a model of well-differentiated human airway epithelium (WD-HAE) that exhibited robust FcRn expression, and measured the transepithelial transport of a mAb against SARS-CoV-2 Spike protein (CR3022) with either wildtype IgG₁-Fc or Fc modified with YTE or LS mutations known to increase affinit

SUBMITTER: Togami K 

PROVIDER: S-EPMC11439726 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature