GenoMine: a CRISPR-Cas9-based kill switch for biocontainment of Pseudomonas putida.

ABSTRACT: Synthetic genetic circuits have revolutionised our capacity to control cell viability by conferring microorganisms with programmable functionalities to limit survival to specific environmental conditions. Here, we present the GenoMine safeguard, a CRISPR-Cas9-based kill switch for the biotechnological workhorse Pseudomonas putida that employs repetitive genomic elements as cleavage targets to unleash a highly genotoxic response. To regulate the system's activation, we tested various circuit-based mechanisms including the digitalised version of an inducible expression system that operates at the transcriptional level and different options of post-transcriptional riboregulators. All of them were applied not only to directly control Cas9 and its lethal effects, but also to modulate the

SUBMITTER: Asin-Garcia E 

PROVIDER: S-EPMC11439788 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature