Identification and validation of ferroptosis-related biomarkers in intervertebral disc degeneration.

ABSTRACT:

Introduction

Ferroptosis plays a significant role in intervertebral disc degeneration (IDD). Understanding the key genes regulating ferroptosis in IDD could reveal fundamental mechanisms of the disease, potentially leading to new diagnostic and therapeutic targets.

Methods

Public datasets (GSE23130 and GSE70362) and the FerrDb database were analyzed to identify ferroptosis-related genes (DE-FRGs) involved in IDD. Single-cell RNA sequencing data (GSE199866) was used to validate the specific roles and expression patterns of these genes. Immunohistochemistry and Western blot analyses were subsequently conducted in both clinical samples and mouse models to assess protein expression levels across different tissues.

Results

The analysis identified seven DE-FRGs, including <

SUBMITTER: Li C 

PROVIDER: S-EPMC11439793 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature