Uncovering the Penile Clock: Expression of Molecular Clock Proteins in Human Penile Cavernous Tissue.

ABSTRACT:

Purpose

To evaluate the expression of core molecular clock genes/proteins in penile cavernous tissue from healthy male subjects and to determine whether their expression has circadian variation.

Materials and methods

Corpus cavernosum biopsy samples were obtained from 10 healthy males with penile deviation or fracture who underwent surgical intervention during the day and night. The daytime group (n=5) underwent corpus cavernosum tissue sampling during zeitgeber time (ZT) 8-12, while the nighttime group (n=5) underwent sampling during ZT 20-24. The expression and localization of BMAL1, CLOCK, PER1, PER2, PER3, CRY1, and CRY2 proteins were analyzed using immunohistochemistry and quantified using H-score analysis. RT-qPCR analysis was performed to assess the expression of core

SUBMITTER: Alkan I 

PROVIDER: S-EPMC11439802 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature