Ferroptosis Is Crucial for Cisplatin Induced Sertoli Cell Injury <i>via</i> N6-Methyladenosine Dependent Manner.

ABSTRACT:

Purpose

This study aimed to investigate the effect of the N6-methyladenosine (m6A) dependent ferroptosis on cisplatininduced Sertoli cell injury.

Materials and methods

A cisplatin exposure mouse model was established by intraperitoneal injection of cisplatin in our study. TM4 cell lines was used for in vitro study. Ferroptosis was detected according to metabolomic analysis and a series of assays, including malondialdehyde, glutathione, and glutathione disulfide concentration detection, 2',7'-dichlorodihydrofluorescein diacetate and BODIPY 581/591 C11 probe detection, and transmission electron microscope imaging. Key ferroptosis-related genes were identified via transcriptomic analysis, western blot and immunohistochemistry. The m6A modification was demonstrated

SUBMITTER: Fan Z 

PROVIDER: S-EPMC11439804 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature