HECTD2 as a target for veratric acid in the regulation of ferroptosis in renal cell carcinoma.

ABSTRACT: Function of HECTD2 in renal cell carcinoma malignant progression is undefined. Molecular mechanism behind anti-cancer effects of veratric acid (VA) from traditional Chinese medicine (TCM) is underexplored. The Cancer Genome Atlas was leveraged to study HECTD2 expression in renal cell carcinoma and its relationship with histological grading. Kaplan-Meier survival analysis was performed. HECTD2 expression was detected in cancer cells and tissues via qRT-PCR and immunohistochemistry. GPX4 and SLC7A11 expression in tumor samples with high or low HECTD2 expression was examined by immunohistochemistry, cell viability by CCK8, cell proliferation by colony formation assay, lipid ROS and mitochondrial superoxide levels by flow cytometry, Fe²⁺ and MDA content by assay kits, and GPX4 and S

SUBMITTER: Lv D 

PROVIDER: S-EPMC11439856 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature