PTMoreR-enabled cross-species PTM mapping and comparative phosphoproteomics across mammals.

ABSTRACT: To support PTM proteomic analysis and annotation in different species, we developed PTMoreR, a user-friendly tool that considers the surrounding amino acid sequences of PTM sites during BLAST, enabling a motif-centric analysis across species. By controlling sequence window similarity, PTMoreR can map phosphoproteomic results between any two species, perform site-level functional enrichment analysis, and generate kinase-substrate networks. We demonstrate that the majority of real P-sites in mice can be inferred from experimentally derived human P-sites with PTMoreR mapping. Furthermore, the compositions of 129 mammalian phosphoproteomes can also be predicted using PTMoreR. The method also identifies cross-species phosphorylation events that occur on proteins with an increased tendency to re

SUBMITTER: Wang S 

PROVIDER: S-EPMC11440062 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature