Advancing drug safety and mitigating health concerns: High-resolution mass spectrometry in the levothyroxine case study.

ABSTRACT: Levothyroxine is a drug with a narrow therapeutic index. Changing the drug formulation composition or switching between pharmaceutical brands can alter the bioavailability, which can result in major health problems. However, the increased adverse drug reactions have not been fully explained scientifically yet and a thorough investigation of the formulations is needed. In this study, we used a non-targeted analytical approach to examine the various levothyroxine formulations in detail and to reveal possible chemical changes. Ultra-high-performance liquid chromatography coupled with a data-independent acquisition high-resolution mass spectrometry (UHPLC-DIA-HRMS) was employed. UHPLC-DIA-HRMS allowed not only the detection of levothyroxine degradation products, but also the presence of non-ex

SUBMITTER: Chmelarova H 

PROVIDER: S-EPMC11440252 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature