ER-mitochondria contact sites regulate hepatic lipogenesis via Ip3r-Grp75-Vdac complex recruiting Seipin.

ABSTRACT:

Background

Mitochondria and endoplasmic reticulum (ER) contact sites (MERCS) constitute a functional communication platform for ER and mitochondria, and they play a crucial role in the lipid homeostasis of the liver. However, it remains unclear about the exact effects of MERCs on the neutral lipid synthesis of the liver.

Methods

In this study, the role and mechanism of MERCS in palmitic acid (PA)-induced neutral lipid imbalance in the liver was explored by constructing a lipid metabolism animal model based on yellow catfish. Given that the structural integrity of MERCS cannot be disrupted by the si-mitochondrial calcium uniporter (si-mcu), the MERCS-mediated Ca²⁺ signaling in isolated hepatocytes was intercepted by transfecting them with si-mcu in some in vitro ex

SUBMITTER: Chi YJ 

PROVIDER: S-EPMC11440722 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature