De novo biosynthesis of β-Arbutin in Komagataella phaffii based on metabolic engineering strategies.

ABSTRACT:

Background

β-Arbutin, found in the leaves of bearberry, stands out as one of the globally acknowledged eco-friendly whitening additives in recent years. However, the natural abundance of β-Arbutin is low, and the cost-effectiveness of using chemical synthesis or plant extraction methods is low, which cannot meet the requirements. While modifying the β-Arbutin synthesis pathway of existing strains is a viable option, it is hindered by the limited synthesis capacity of these strains, which hinders further development and application.

Results

In this study, we established a biosynthetic pathway in Komagataella phaffii for β-Arbutin production with a titer of 1.58 g/L. Through diverse metabolic strategies, including fusion protein construction, enhancing shikimate pathway flux,

SUBMITTER: Yang J 

PROVIDER: S-EPMC11440761 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature