Single-cell analysis of preimplantation embryonic development in guinea pigs.

ABSTRACT:

Background

Guinea pigs exhibit numerous physiological similarities to humans, yet the details of their preimplantation embryonic development remain largely unexplored.

Results

To address this, we conducted single-cell sequencing on the transcriptomes of cells isolated from the zygote stage through preimplantation stages in guinea pigs. This study identified seven distinct cell types within guinea pig preimplantation embryos and pinpointed the timing of zygotic gene activation (ZGA). Trajectory analysis revealed a bifurcation into two lineage-specific branches, accompanied by alterations in specific pathways, including oxidative phosphorylation and vascular endothelial growth factor (VEGF). Additionally, co-expressed gene network analysis highlighted the most enriched functio

SUBMITTER: Guan T 

PROVIDER: S-EPMC11440810 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature