Mediator complex subunit 1 promotes oral squamous cell carcinoma progression by activating MMP9 transcription and suppressing CD8⁺ T cell antitumor immunity.

ABSTRACT:

Background

The role of Mediator complex subunit 1 (MED1), a pivotal transcriptional coactivator implicated in diverse biological pathways, remains unexplored in the context of oral squamous cell carcinoma (OSCC). This study aims to elucidate the contributory mechanisms and potential impact of MED1 on the progression of OSCC.

Methods

The expression and clinical significance of MED1 in OSCC tissues were evaluated through the bioinformatics analyses. The effects of MED1 on the biological behavior of OSCC cancer cells were assessed both in vitro and in vivo. Dual-luciferase reporter assay, chromatin immunoprecipitation (ChIP) assay, bioinformatic analysis, CD8⁺ T cell isolation experiment, coculture experiment, enzyme-linked immunosorbent assay (ELISA), and flow cytom

SUBMITTER: Li Z 

PROVIDER: S-EPMC11440895 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature