Implications of accumulation of clonally expanded and senescent CD4⁺GNLY⁺ T cells in immunological non-responders of HIV-1 infection.

ABSTRACT: Increased CD4⁺GNLY⁺ T cells have been confirmed to be inversely associated with CD4⁺ T cell count in immunological non-responders (INRs), however, the underlying mechanisms are unknown. This study aimed to elucidate the characteristics of CD4⁺GNLY⁺ T cells and their relationship with immune restoration. Single-cell RNA sequencing, single-cell TCR sequencing, and flow cytometry were used to analyze the frequency, phenotypes, and function of CD4⁺GNLY⁺ T cells. Moreover, Enzyme linked immunosorbent assay was performed to detect plasma cytokines production in patients. CD4⁺GNLY⁺ T cells were found to be highly clonally expanded, characterized by higher levels of cytotoxicity, senescence, P24, and

SUBMITTER: Yang X 

PROVIDER: S-EPMC11441045 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature