RGeasy: a reference gene analysis tool for gene expression studies via RT-qPCR.

ABSTRACT: Gene expression through RT-qPCR can be performed by the relative quantification method, which requires the expression normalization through reference genes. Therefore, it is essential to validate, experimentally, the candidate reference genes. Thus, although there are several studies that are performed to identify the most stable reference genes, most them validate genes for very specific conditions, not exploring the whole potential of the research since not all possible combinations of treatments and/or conditions of the study are explored. For this reason, new experiments must be conducted by researchers that have interest in analyzing gene expression of treatments and/or conditions present, but not explored, in these studies. Here, we present the RGeasy tool, which aims to facilitate t

SUBMITTER: de Souza MR 

PROVIDER: S-EPMC11441100 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature