In vivo CRISPR screens identify Mga as an immunotherapy target in triple-negative breast cancer.

ABSTRACT: Immune evasion is not only critical for tumor initiation and progression, but also determines the efficacy of immunotherapies. Through iterative in vivo CRISPR screens with seven syngeneic tumor models, we identified core and context-dependent immune evasion pathways across cancer types. This valuable high-confidence dataset is available for the further understanding of tumor intrinsic immunomodulators, which may lead to the discovery of effective anticancer therapeutic targets. With a focus on triple-negative breast cancer (TNBC), we found that Mga knock-out significantly enhances antitumor immunity and inhibits tumor growth. Transcriptomics and single-cell RNA sequencing analyses revealed that Mga influences various immune-related pathways in the tumor microenvironment. Our findin

SUBMITTER: Feng X 

PROVIDER: S-EPMC11441491 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature