Measurement of adhesion and traction of cells at high yield reveals an energetic ratchet operating during nephron condensation.

ABSTRACT: Developmental biology-inspired strategies for tissue-building have extraordinary promise for regenerative medicine, spurring interest in the relationship between cell biophysical properties and morphological transitions. However, mapping gene or protein expression data to cell biophysical properties to physical morphogenesis remains challenging with current techniques. Here, we present multiplexed adhesion and traction of cells at high yield (MATCHY). MATCHY advances the multiplexing and throughput capabilities of existing traction force and cell-cell adhesion assays using microfabrication and a semiautomated computation scheme with machine learning-driven cell segmentation. Both biophysical assays are coupled with serial downstream immunofluoresce

SUBMITTER: Liu J 

PROVIDER: S-EPMC11441508 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature