Combining mutation and recombination statistics to infer clonal families in antibody repertoires.

ABSTRACT: B-cell repertoires are characterized by a diverse set of receptors of distinct specificities generated through two processes of somatic diversification: V(D)J recombination and somatic hypermutations. B-cell clonal families stem from the same V(D)J recombination event, but differ in their hypermutations. Clonal families identification is key to understanding B-cell repertoire function, evolution, and dynamics. We present HILARy (high-precision inference of lineages in antibody repertoires), an efficient, fast, and precise method to identify clonal families from single- or paired-chain repertoire sequencing datasets. HILARy combines probabilistic models that capture the receptor generation and selection statistics with adapted clustering methods to achieve consistently high inference accura

SUBMITTER: Spisak N 

PROVIDER: S-EPMC11441979 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature