Gut microbiota and metabolomic profile changes play critical roles in tacrolimus-induced diabetes in rats.

ABSTRACT:

Aims

Hyperglycemia is one of the adverse effects of tacrolimus (TAC), but the underlying mechanism is not fully identified. We used multi-omics analysis to evaluate the changes in the gut microbiota and metabolic profile of rats with TAC-induced diabetes.

Methods

To establish a diabetic animal model, Sprague Dawley rats were divided randomly into two groups. Those in the TAC group received intraperitoneal injections of TAC (3 mg/kg) for 8 weeks, and those in the CON group served as the control. 16S rRNA sequencing was used to analyze fecal microbiota. The metabolites of the two groups were detected and analyzed by nontargeted and targeted metabolomics, including amino acids (AAs), bile acids (BAs), and short-chain fatty acids (SCFAs).

Results

The rats treated with TAC

SUBMITTER: Jiang Z 

PROVIDER: S-EPMC11442430 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature